Part:BBa_K5403010

Membrane protein RbsC missing 35 C-terminal amino acids

The mycobacterial protein RbsC is a ribose transport system permease protein and was shown to be part of the vesicles of M. smegmatis (Prados-Rosales et al., 2011). The protein is documented in the mycobacterial database MyCoBrowser (MyCoBrowser, MSMEG_1710).

This part was designed as a domain for the composite part BBa_K5403019, which is a fusion protein of truncated RbsC, a flexible linker and GFP, aiming to anchor the GFP into the membrane of the vesicles of M. smegmatis. The reason for the truncation at the C-terminus is to make sure that the C terminus is located at the periplasmic side of the membrane, which is the side that GFP should be displayed on.

References

MyCoBrowser. (n.d.). https://mycobrowser.epfl.ch/genes/MSMEG_1710 Prados-Rosales, R., Baena, A., Martinez, L. R., Luque-Garcia, J., Kalscheuer, R., Veeraraghavan, U., Camara, C., Nosanchuk, J. D., Besra, G. S., Chen, B., Jimenez, J., Glatman-Freedman, A., Jacobs, W. R., Porcelli, S. A., & Casadevall, A. (2011). Mycobacteria release active membrane vesicles that modulate immune responses in a TLR2-dependent manner in mice. Journal Of Clinical Investigation, 121(4), 1471–1483. https://doi.org/10.1172/jci44261

Prados-Rosales, R., Baena, A., Martinez, L. R., Luque-Garcia, J., Kalscheuer, R., Veeraraghavan, U., Camara, C., Nosanchuk, J. D., Besra, G. S., Chen, B., Jimenez, J., Glatman-Freedman, A., Jacobs, W. R., Porcelli, S. A., & Casadevall, A. (2011). Mycobacteria release active membrane vesicles that modulate immune responses in a TLR2-dependent manner in mice. Journal Of Clinical Investigation, 121(4), 1471–1483. https://doi.org/10.1172/jci44261

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 146
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 146
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 146
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 146
  Illegal AgeI site found at 712
  
• 1000
  COMPATIBLE WITH RFC[1000]